The Kampmann lab used CRISPRi from a safe-harbor site (SHS), a locus in the genome where genes can be safely inserted and not disrupt other genes, to decrease neuron growth without cutting their DNA. More specifically, desired genes were repressed at different points in neuronal differentiation.

Martin KAMPMANN, Assistant Professor of University of California, San Francisco CRISPRi has minimal impact on ATP levels under basal conditions during which both respiration and glycolysis are

Such neurons are powerful tools to study cellular mech-anisms of neurodegenerative diseases. iPSCs 2018-04-13 · CRISPRi and CRISPRa: New Functional Genomics Tools Provide Complementary Insights into Cancer Biology and Therapeutic Strategies Martin Kampmann, Ph.D. Examples of cancer cell vulnerabilities include driver oncogenes that are essential for the initiation and progression of cancer, or non-oncogene addictions resulting from the cancerous state of the cell. 2020-10-20 · Kampmann used the technique to understand which genes are essential for neurons to survive and deal with stress.

Specifically, Kampmann will: Cover CRISPRi (interference) and CRISPRa (activation) control of gene expression. Describe how to implement CRISPRi/a in iPSC-derived cells. Introduce large-scale genetic screens based on survival, fluorescent readouts of cell function, single-cell RNA sequencing, and high-content imaging. Kampmann explains: "For CRISPRi, we target a transcriptional repressor domain (the KRAB domain) to the transcription start site of genes to repress their expression. This knockdown approach is highly effective and lacks the notorious off-target effects of RNAi-based gene knockdown." CRISPRbrain is an amazing resource for functional genomics screens in human cell types. This data commons is laying the #openscience foundation for a ton of high-throughput unbiased screens in the future.

2020-03-04 · Analysis of CRISPRi screen results was carried out using custom code (MAGeCK-iNC) developed in the Kampmann lab, which was previously described 37 and is freely available at https://kampmannlab

We introduce the underlying technology and present different types of CRISPRi/a screens, including those based on cell survival/proliferation, sensitivity to drugs or toxins, fluorescent reporters, and single-cell transcriptomes. CRISPRi and CRISPRagenetic screening inhumaniPSC-derivedneurons.CRISPRi in iPSCs has previously been demon-strated [8], and our own unpublished results have recently established the fea-sibility of pooled CRISPRi-based screens in iPSC-derived neurons. Such neurons are powerful tools to study cellular mech-anisms of neurodegenerative diseases.

In a project led by postdoc Xiaoyan Guo in the Kampmann lab, a CRISPRi-based genetic screen uncovered the molecular mechanism by which mitochondrial dysfunction is relayed to the rest of the cell. The mitochondrial protease OMA1 cleaves a previously little characterized protein, DELE1.

Percent Expression of Target Genes in A375 CRISPRi Cells. Figure 1. Panning , Hidde L. Ploegh, Michael C. Bassik, Lei S. Qi, Martin Kampmann, Jonathan S. SFARI | Martin Kampmann on SFARI. cells, which incorporates systematic genetic interaction maps and CRISPR-based gain- and loss-of-function screens. olivia teter.

Genetic screens in human-induced pluripotent stem cell-derived neurons can elucidate such mechanisms. Genome-wide screens using CRISPR interference … Cancer cells commonly develop resistance to immunotherapy by loss of antigen expression.
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M. Kampmann , CRISPRi and CRISPRa Screens in Mammalian Cells for Precision Biology and Medicine. . ACS Chem. Biol.

The mitochondrial protease OMA1 cleaves a previously little characterized protein, DELE1. Martin Kampmann, Ph.D. A central goal of research for targeted cancer therapy, or precision oncology, is to reveal the intrinsic vulnerabilities of cancer cells and exploit them as therapeutic targets.
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2020-09-24

Experiment. Twelve 10-cm Matrigel-coated dishes were each seeded with 4*106 CRISPRi-iPSCs infected with virus for the H1 CRISPRi-v2 sgRNA library in N2 Pre-Differentiation Medium (day -3) and differentiated by doxycyclin-induced Ngn2 expression.

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2020-03-04 · Analysis of CRISPRi screen results was carried out using custom code (MAGeCK-iNC) developed in the Kampmann lab, which was previously described 37 and is freely available at https://kampmannlab

Neurodegenerative, neurodevelopmental and neuropsychiatric disorders are among the greatest public health challenges, Development of a CRISPR screening platform in microglia to elucidate Chao Wang2, Claire Clelland2, Wayne W Poon3, Li Gan2, Martin Kampmann1 16 Apr 2020 Khairunnisa Mentari Semesta · Ruilin Tian · Martin Kampmann · Mark von Zastrow · Nikoleta G. Tsvetanova. Kampmann: Multiplexing with CRISPR Screens. July 15, 2016 Genome Engineering, Video, Lecture.